Flow Cytometric Measurement of Hemoglobin F in RBCs

Flow Cytometric Measurement of Cell Organelle Autophagy

Flow cytometric measurement of calpain activity in living cells.

BACKGROUND Calpains are intracellular, calcium-sensitive, neutral cysteine proteases that play crucial roles in many physiological and pathological processes. Calpain regulation is complex and activity is poorly correlated with calpain protein levels. Therefore a full understanding of calpain function requires robust methods for measuring activity. METHODS We describe and characterize a flow ...

Flow Cytometric Measurement of CD41/CD61, CD42b Platelet Receptors and Platelet Factor 3 Activity in Lyophilized Infusible Platelet Membrane Preparation

Background: The short life time of human platelet units has led to a chronic shortage of fresh platelets in blood transfusion centers. Many approaches have been investigated experimentally to produce new hemostatically active platelet products that are capable of long term storage. Infusible platelet membrane (IPM) prepared from fresh or outdated human platelets have been developed as an altern...

Rapid flow cytometric measurement of protein inclusions and nuclear trafficking

Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both s...

Hemoglobin F level in different hemoglobin variants

BACKGROUND Fetal hemoglobin (HbF) levels in different hemoglobin variants in Osogbo, Nigeria, were estimated using two principal methods of estimation using existing information for HbF concentration and distribution of various hemoglobin variants in the area, as well as diagnosed cases of thalassemia. Two hundred and sixty samples collected from HbSS, HbSC, HbAA, HbAS, and HbAC subjects were a...